Properties and synthesis methods of guanidine isothiocyanate (593-84-0)
Jun 14, 2024| Properties and synthesis methods of guanidine isothiocyanate (593-84-0)
Nature
Guanidine thiocyanate has good solubility, soluble in water and ethanol, but is sensitive to light and irritating.
Characteristics and advantages
A versatile surfactant suitable for cell biology and biochemistry research
Suitable for sensitive molecular biological applications for RNA extraction
Does not contain DNA enzyme and RNA enzyme
The test confirmed that the heavy metal pollution level is low and the applicability is wide
Use
Guanidine thiocyanate is a high purity biochemical reagent, which is widely used in the field of molecular biology. It does not contain detectable nuclease and protease activity, and is also a powerful protein denaturant because both its cations and anions are ionizing agents.
Effect
Guanidine thiocyanate is an uncoupling agent that dissolves proteins and is mainly used to lyse cells and release proteins and nucleic acid materials within cells to extract RNA and DNA.
Synthesis method
The synthesis method of guanidine thiocyanate includes the following processes:
(1) Put guanidine carbonate and ammonium sulfate into the reaction kettle according to the weight ratio of 2:1, and react at the appropriate temperature for a certain time to obtain guanidine thiocyanate crude.
(2) The guanidine thiocyanate is dissolved in water at a ratio of 0.5:1 and dissolved at room temperature.
(3) Impurities such as raw materials and reaction by-products are removed by filtration.
(4) Dehydrate the filtered mother liquor at high temperature.
(5) The solution is cooled and concentrated and crystallized to obtain high-purity guanidine thiocyanate.