CAS：593-85-1|Application of guanidine carbonate in the field of biochemistry

CAS：593-85-1|Application of guanidine carbonate in the field of biochemistry

Source: Reprinted from third party

Product name: Guanidine carbonate

Alias: carbamide urea, guanidine carbonate

English name: Guanidine carbonate

CAS: 593-85-1

Molecular formula: C3H12N6O3

Item number: T0055

Structural formula:

Guanidine carbonate application field

Nucleic acid extraction and purification is a basic operation in molecular biology. At present, in molecular biology or clinical gene diagnosis, nucleic acid extraction by centrifugal column method and magnetic bead method has high purity, and can realize automatic operation, and has the advantages of efficient extraction, convenient extraction and large throughput, which is the development direction of nucleic acid extraction in clinical diagnosis in the future. In actual operation, due to the effect of guanidine salt to reduce the pH value of the solution, the guanidine salt in these cell lysates is easy to precipitate when the pH value is near neutral or acidic, which not only brings the lack of reagents needing to be heated and dissolved and the inconvenient operation of absorption, but also affects the cell lysation effect in the sample and reduces the yield of nucleic acid extraction. Therefore, CN102533724B patent provides a cell lysis reagent for nucleic acid extraction and purification of biological samples, the main components are guanidine salt, ammonium ion and surfactant, among which guanidine salt can be guanidine isothiocyanate, guanidine hydrochloride, guanidine carbonate, guanidine phosphate, etc.

The guanidine salt in the patented reagent is a strong denaturant of protein and nucleic acid, and an accelerator for binding and adsorption of nucleic acid and silica material solid phase material, especially due to the synergistic effect of the selected key component ammonium ion and surfactant. It can not only fully lyse cells, separate denatured nucleic acid molecules that have lost secondary structure from denatured protein molecules, but also maintain the stability of nucleic acid in the lysate mixture, and can be widely used in the extraction and purification of nucleic acid from different types of biological samples such as serum, plasma, saliva and urine.

Reference

CN102533724B A cell lysis reagent for the extraction and purification of nucleic acids from biological samples.